Second, thrombus formation was also induced by direct endothelial cell disruption by femto-second laser irradiations. Discoid platelet activations and aggregations were induced without leukocyte recruitment in this system. Results: First, we induced rapidly developing thrombi composed of discoid platelets, which was triggered by ROS production without endothelial damage. Methods: We combined multi-photon microscope technique, and lightmanipulations system, which enabled us to evaluate platelet aggregations by photochemical reactions. Aims: To elucidate blood cell behavior and vascular responses, we improved in vivo imaging technique based on multi-photon microscopy, and analyzed new three animal thrombus formation models. ABSTRACTS OF THE 62ND ANNUAL MEETING OF THE SCIENTIFIC AND STANDARDIZATION COMMITTEE OF THE INTERNATIONAL SOCIETY ON THROMBOSIS AND HAEMOSTASIS MAY 25–28, 2016 Animal, Cellular and Molecular Models AMC01 Endothelial injury and hypoxia enhances neutrophil recruitment, extravasation, and apoptosis during thrombus formation Nishimura S1, Seo K2 and Sakata A2 1 Jichi Med Univ, and the Univ of Tokyo, Tochigi, Japan 2Jichi Med Univ, Tochigi, Japan Background: Primary trigger of cardiovascular events remains unclear due to the absence of direct analysis tools of in vivo thrombus formation.
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